Method for improving germline transmission efficiency of avian primordial germ cells
US7476776B2 · kind B2 · utility
10Cited by
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8Claims
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Key dates
| Filing date | Apr 21, 2004 |
| Grant date | Jan 13, 2009 |
| Priority date | — |
| Expiry date | Apr 21, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2517/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a method for improving the germline transmission efficiency of avian primordial germ cells (PGCs), and methods for producing avian chimeras and transgenic using it. The present method comprises the steps of (a) isolating primordial germ cells (PGCs) from an avian embryonic gonad; and (b) culturing said PGCs in vitro for at least 5 days. According to the present method, the germline transmission efficiency of PGCs can be dramatically improved in a feasible manner.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.