Triacylglycerol-deficient fission yeast and its uses
US7479374B2 · kind B2 · utility
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Key dates
| Filing date | Jul 9, 2004 |
| Grant date | Jan 20, 2009 |
| Priority date | — |
| Expiry date | Jul 20, 2025 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/025
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A fission yeast having non-functional dga1 and plh1 genes, which is incapable of synthesizing triacylglycerols, is disclosed. The fission yeast is susceptible to lipotoxicity including lipoapoptosis, and is useful for screening of compounds that inhibit or prevent lipotoxicity or lipoapoptosis. The fission yeast strain may be transformed to express a mammalian enzyme involved in triacylglycerol synthesis, and therefore is also useful for screening of compounds that inhibit or prevent triacylglycerol synthesis, which activity is conferred by the mammalian enzyme.
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