Deglycosylated enzymes for conjugates

US7482145B2 · kind B2 · utility

Assignee

• Roche Diagnostics Operations, Inc. · US

Inventors

• Wolfgang Hoesel · Tutzing, DE
• Werner Hoelke · Penzberg, DE
• Beata Kanne · Schwanau, DE
• Helmut Lenz · Tutzing, DE
• Rainer Mueller · Penzberg, DE

Key dates

• Filing date: Sep 15, 2005
• Grant date: Jan 27, 2009
• Expiry date: Sep 15, 2025

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/535
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

By deglycosylating an enzyme derived from a eukaryotic organism prior to forming a conjugate, i.e. prior to attaching the enzyme to a molecule capable of binding to a target molecule, the sensitivity of an assay for detecting the presence or determining the quantity of a target molecule, in which the conjugate is used as a labelled component, can be increased. The present invention provides conjugates, methods for providing the same, use of such conjugates as well as kits containing the conjugates.