Monacolin K biosynthesis genes
US7482157B2 · kind B2 · utility
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16Claims
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Key dates
| Filing date | Dec 23, 2004 |
| Grant date | Jan 27, 2009 |
| Priority date | — |
| Expiry date | Apr 16, 2026 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y101/01088
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Cholesterol inhibitor produced by Monascus, monacolin k, is a secondary metabolite of polyketides. The invention provides probes specific to monacolin k biosynthesis gene cluster. BAC clones having putative monacolin k gene cluster were screened from BAC (bacterial artificial chromosome) library, and sequencing and annotation were performed on these clones. The results show that 2 polyketide synthase (PKS) genes and 7 regulatory genes related to monacolin k synthesis were obtained. Full-length cDNAs of these genes were then obtained by RT-PCR and cloned to expression vectors for the expression of these genes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.