Patent · US Active

Method of preventing white blood cell interferences to red blood cell measurements of a blood sample

US7482165B2 · kind B2 · utility

7Cited by
16References
21Claims
0Family size

Assignee

Inventors

Key dates

Filing dateAug 24, 2005
Grant dateJan 27, 2009
Priority date
Expiry dateMay 15, 2027

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10T436/25125
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

Methods of prevention and correction of white blood cell interferences to the measurements of mean cell volume (MCV), red blood cell concentration (RBC) and hematocrit (Hct) of a blood sample are disclosed. The interference to MCV can be prevented by identifying a valley between the red blood cell and white blood cell modes on the red blood cell distribution histogram, defining a red blood cell region using the valley and calculating MCV within the defined region. Alternatively, a curve fit of the white blood cell population on the red blood cell distribution histogram can be used to exclude the white blood cells. RBC can be corrected by subtracting the white blood cell concentration obtained from the analysis of a second aliquot sample, when the white blood cell concentration (WBC) exceeds a predetermined criterion. Hct of the blood sample can be calculated using the obtained MCV and RBC.

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