Ladder assembly and system for generating diversity
US7485423B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 11, 2005 |
| Grant date | Feb 3, 2009 |
| Priority date | — |
| Expiry date | Apr 15, 2026 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6844
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides novel methods of generating a nucleic acid molecule. In certain embodiments, a double stranded nucleic acid chunk is generated from a ladder complex comprising partially complementary oligonucleotides, which chunk is combined with a nucleic acid acceptor molecule. In certain embodiments, the assembled chunk/nucleic acid acceptor molecule complex may be propagated in vivo or in vitro. The present invention also provides improved systems for generating a plurality of nucleic acid molecules that differ at one or more nucleotide positions. In certain embodiments, the plurality of nucleic acid molecules encodes a polypeptide or portion of a polypeptide.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.