Recombinase polymerase amplification
US7485428B2 · kind B2 · utility
82Cited by
7References
12Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 13, 2007 |
| Grant date | Feb 3, 2009 |
| Priority date | — |
| Expiry date | Aug 13, 2027 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG06F16/9574
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This disclosure describe three related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of the bacterial RecA and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods has the advantage of not requiring thermocycling or thermophilic enzymes. Further, the improved processivity of the disclosed methods allow amplification of DNA up to hundreds of megabases in length.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.