Simultaneous detection of metabolic enzyme activity and metabolite levels
US7485435B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Feb 28, 2005 |
| Grant date | Feb 3, 2009 |
| Priority date | — |
| Expiry date | Feb 28, 2025 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2800/04
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Provided are methods for detecting a metabolic disorder in an individual using mass spectrometry. One method involves (a) contacting a sample containing (i) one or more metabolically indicative enzymes and (ii) one or more metabolic analytes, with one or more substrates for said one or more enzymes to produce a reaction admixture, under conditions wherein at least one of said enzymes is capable of acting on a corresponding substrate to generate at least one product, and wherein one or more protease inhibitors are present; (b) contacting said reaction admixture with a reagent that inhibits the ability of said one or more enzymes to act on a corresponding substrate, wherein said one or more metabolic analytes and said at least one product are soluble in said reagent; to produce a test sample and (c) determining the presence or amount of said one or more metabolic analytes and said at least one product contained in said test sample using mass spectrometry, wherein a determined presence or amount of said one or more metabolic analytes and said at least one product correlates with presence or absence of said metabolic disorder. product correlates with presence or absence of said metabol…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.