Patent · US Expired

Method for detecting DNA point mutations (single nucleotide polymorphism (SNP) analysis) and associated arrangement

US7488578B2 · kind B2 · utility

9Cited by

4References

23Claims

0Family size

Assignee

Siemens Aktiengesellschaft · DE

Inventors

Walter Gumbrecht · Herzogenaurach, DE
Peter Paulicka · Erlangen, DE
Manfred Stanzel · Erlangen, DE

Key dates

Filing date	May 28, 2004
Grant date	Feb 10, 2009
Priority date	—
Expiry date	Jun 12, 2024

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/6837
WIPO fieldChemical engineering
WIPO sectorChemistry

Abstract

The single nucleotide polymorphism analysis involves the utilization of a DNA hybridization process as well as the use of a DNA chip. A liquid DNA sample to be analyzed is guided over a DNA chip in a defined time course. After successful hybridization, the temperature is modified in a defined manner under low stringency conditions such that scavenger/target DNA hybrids are melted, whereby the melting of the scavenger/target DNA hybrids is detected and evaluated according to the temperature. In addition to the DNA chip, at least one device is provided that controls and regulates the temperature, and another device is provided that controls a lateral flow of liquid against the surface of the DNA chip. Factors for matching hybrids and mismatching/single point mismatching hybrids can be detected and evaluated using appropriate measuring device(s).

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.