Patent · US Active

Adsorption of nucleic acids to a solid phase

US7491495B2 · kind B2 · utility

25Cited by

15References

19Claims

0Family size

Assignee

Roche Diagnostics Operations, Inc. · US

Inventors

Ralf Zielenski · Benediktbeuern, DE
Klaus Geissler · Backnang, DE
Thomas Walter · Penzberg, DE

Key dates

Filing date	Feb 18, 2005
Grant date	Feb 17, 2009
Priority date	—
Expiry date	Dec 11, 2026

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/6834
WIPO fieldOrganic fine chemistry
WIPO sectorChemistry

Abstract

The present invention is directed to a method for adsorbing, i.e. non-covalently binding, nucleic acids to a solid phase using a two-step procedure. Furthermore, the present invention pertains to a method for isolating nucleic acids from a biological sample. In the first step of the procedure, lysis is effected by mixing the biological sample with an aqueous lysis buffer containing a chaotropic agent and incubating the mixture; in the second step, the concentration of the chaotropic agent in the mixture is increased and the mixture is contacted with the solid phase, whereby the nucleic acids in the liquid phase is adsorbed to the solid phase.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.