Patent · US Expired

Universal method and composition for the rapid lysis of cells for the release of nucleic acids and their detection

US7494771B2 · kind B2 · utility

42Cited by
9References
45Claims
0Family size

Assignee

Inventors

Key dates

Filing dateJul 19, 2002
Grant dateFeb 24, 2009
Priority date—
Expiry dateFeb 25, 2023

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q1/6806
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

This invention describes a rapid (10 to 15 minutes), simple, flexible and efficient method of nucleic acids extraction for nucleic acid testing assays. This method has the following basic steps: i) mechanical cell lysis using solid particles in the presence of a chelating agent, followed by ii) controlling the presence and/or activity of NAT assays inhibitors. This method is applicable to various biological samples and universal for microorganisms, as one can use it to extract nucleic acids from test samples containing target viruses, bacteria, bacterial spores, fungi, parasites or other eukaryotic cells, including animal and human cells.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.