Recombinant terminal deoxynucleotidyl transferase with improved functionality

US7494797B2 · kind B2 · utility

Assignee

• Roche Diagnostics Operations, Inc. · US

Inventors

• Rainer Mueller · Penzberg, DE
• Markus Pajatsch · Munich, DE
• Ingo Curdt · Hochheim, DE
• Harald Sobek · Penzberg, DE
• Manfred Schmidt · Krefeld, DE
• Bernhard Suppmann · Weilheim, DE
• Kirsten Sonn · Penzberg, DE
• Bernd Schneidinger · Starnberg, DE

Key dates

• Filing date: Apr 3, 2003
• Grant date: Feb 24, 2009
• Expiry date: Jan 11, 2024

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2510/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Truncated terminal deoxynucleotidyl transferase (TdT) derivative from calf thymus, characterized in that the derivative in comparison to the native TdT is N-terminally truncated by up to 161 amino acids and has a 20- to 30-fold higher enzyme activity in solutions containing Co2+ ions, and its recombinant production and use.