Direct detection of biomolecules in a gel
US7498140B2 · kind B2 · utility
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24Claims
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Key dates
| Filing date | Jul 24, 2006 |
| Grant date | Mar 3, 2009 |
| Priority date | — |
| Expiry date | Oct 19, 2026 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6813
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for detecting a target biomolecule directly in a polyacrylamide gel in which it has been separated from other substances. Prior to binding the target to a probe and while the target biomolecule remains in the gel, the gel is immersed in a water miscible aqueous extracting medium containing a base to shrink the gel by at least about ten percent, and then the gel is washed with water to restore the gel to substantially its original size.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.