Method for cell selection

US7501232B2 · kind B2 · utility

Assignee

• ProBioGen AG · DE

Inventors

• Uwe Marx · Postbauer-Heng, DE
• Christian Demmler · Berlin, DE
• Christoph Giese · Herford, DE

Key dates

• Filing date: May 12, 2004
• Grant date: Mar 10, 2009
• Expiry date: Jan 20, 2026

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/105831
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention relates to a method for the non-invasive selection of single living cells under gentle conditions from mixtures of cells or cell cultures with respect to a specific production performance. To this end, the concentration of substances produced by the individual cells which become enriched at the cell membrane, such as reporter gene products (GFP) or specifically secreted products, such as antibodies, is determined by fluorescence-microscopic detection methods.