Patent · US Active

DNA diagnostics based on mass spectrometry

US7501251B2 · kind B2 · utility

83Cited by

318References

10Claims

0Family size

Assignee

Sequenom, Inc. · US

Inventors

Hubert Koster · Village of La Jolla, US
Andreas Braun · San Diego, US
Dirk Johannes Van Den Boom · Dreieich, DE
Christian Jurinke · Village of La Jolla, US
Andreas Ruppert · Siegsdorf, DE
Franz Hillenkamp · Münster, DE

Key dates

Filing date	Oct 2, 2006
Grant date	Mar 10, 2009
Priority date	—
Expiry date	Oct 2, 2026

Classification

Technology area (CPC H)Electricity
CPC primaryH01J49/00
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

Provided are methods for detecting a target nucleic acid in a biological sample using RNA amplification. In an embodiment, provided is a method that comprises (a) amplifying a target nucleic acid, or portion thereof, using a primer comprising a sequence that is complementary to a polynucleotide sequence in the target nucleic acid, or a complement thereof, and a sequence that encodes an RNA polymerase promoter; (b) synthesizing RNA using an RNA polymerase that recognizes the promoter; and (c) detecting the presence or absence of the resulting RNA using mass spectrometry thereby detecting the presence or absence of the target nucleic acid in the biological sample.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.