Proteorhodopsin mutants with improved optical characteristics
US7501488B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 26, 2003 |
| Grant date | Mar 10, 2009 |
| Priority date | — |
| Expiry date | Mar 4, 2026 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG11B7/2539
- WIPO fieldAudio-visual technology
- WIPO sectorElectrical engineering
Abstract
The present invention is directed to a proteorhodopsin mutant having improved optical characteristics. One improved optical characteristic is having a lower pH (pKrh) at which equal concentrations of the acidic and basic spectral form of the proteorhodopsin molecules are present. Another improved optical characteristic is having a smaller difference in maximum absorption wavelength between the basic and the acidic form. The mutant comprises a mutation in a conserved amino acid residue of a proteorhodopsin variant, which causes spectral shifts. A preferred mutation site is a conserved histidine residue at amino acid position 75 of Bac31A8, or position 77 of Hot75m1, or its equivalent position of a proteorhodopsin variant. Another preferred mutation site is a conserved arginine residue at amino acid position 94 of Bac31A8, or position 96 of Hot75m1, or its equivalent position of a proteorhodopsin variant.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.