Method for improving recovery yield in protein purification with gel filtration chromatography
US7501495B2 · kind B2 · utility
5Cited by
1References
3Claims
0Family size
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Key dates
| Filing date | Mar 3, 2006 |
| Grant date | Mar 10, 2009 |
| Priority date | — |
| Expiry date | Mar 3, 2026 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K16/36
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method to more quantitatively recover a peak of a protein and associated aggregates, hydrophobic proteins, or hydrophobic peptides which are typically difficult to recover and detect as a peak, using a commercially available gel filtration chromatography column and a mobile phase is described. More specifically, a compound, such as arginine, which may be added to the mobile phase, thereby eliminating the unnecessary interaction that occurs between the proteins, peptides, or aggregates and the column stationary phase, is described.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.