Method for characterizing a highly parallelized liquid handling technique using microplates and test kit for carrying out the method

US7504264B2 · kind B2 · utility

Assignee

• Cybio AG · DE

Inventors

• Anton Horn · Jena, DE
• Margarete Schulze · Jena, DE
• Gerhard Cumme · Jena, DE
• Heidrun Rhode · Frankenberg/Sachsen, DE

Key dates

• Filing date: Mar 13, 2003
• Grant date: Mar 17, 2009
• Expiry date: Jun 21, 2025

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/2575
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method for characterizing highly parallelized liquid handling technology using microplates and test kit for carrying out the method. Possibilities for characterizing a multi-channel or many-channel handling technology are known in principle. However, especially with regard to more highly parallelized devices and very small volumes, particularly under conditions corresponding to the actual application conditions, these possibilities were relatively expensive and presented problems especially with respect to the accuracy of evaluation and with respect to correctness and precision. The present method enables a more economical and very exact characterization for applications of this kind. According to the invention, a) a mean sample volume or mean reagent volume is determined by gravimetry from the totality of sample liquid or reagent liquid of all individual channels of the liquid handling technology; b) a normalized mean optical intensity is formed from optical measurement signals of all sample volumes or reagent volumes, each of which is mixed with a diluent; c) the volume accuracy of every individual channel of the liquid handling technology with respect to the mean sample volume…