Transgenic toll-like receptor 9 (TLR9) mice
US7507872B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 5, 2001 |
| Grant date | Mar 24, 2009 |
| Priority date | — |
| Expiry date | May 7, 2024 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA01K2217/075
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention provides a receptor protein specifically recognizing bacterial DNA having an unmethylated CpG sequence, a genomic DNA encoding it, an experimental animal model useful for examining responsiveness of a host immune cell against a bacterial infectious disease. DNA encoding a receptor protein specifically recognizing bacterial DNA having an unmethylated CpG sequence is screened by BLAST search, a number of EST clones having high homology with various TLRs is screened, these clones are used as a probe to isolate a full-length cDNA from mouse macrophage cDNA library, and the sequence of bases of the cDNA is analyzed to confirm that it is TLR9 comprising a conserved regions such as LRR and TIR regions, and then a knockout mouse is produced to confirm that TLR9 is a receptor protein of oligonucleotides having an unmethylated CpG sequence of bacterial DNA.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.