Reagents and a method for saturation labelling of proteins
US7511155B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 8, 2002 |
| Grant date | Mar 31, 2009 |
| Priority date | — |
| Expiry date | Feb 9, 2024 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/6815
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A matched set of fluorescent dyes is provided, wherein each dye of the set is capable of covalent attachment to a protein and wherein each of the dyes has a molecular structure and a charge that is matched one with the other, such that relative electrophoretic mobility of a protein labeled with one dye of the set is the same as the electrophoretic mobility of the protein labeled with a different dye of the set. The matched set comprises at least two different fluorescent dyes of formula:wherein n is 1, 2, or 3; Z1 and Z2 independently represent the carbon atoms necessary to complete a phenyl or naphthyl ring system; one of groups R1 and R2 is a target bonding group; remaining group R1 or R2 is selected from —(CH2)4—W or —(CH2)r—H; group R3 is hydrogen, except when either R1 or R2 is —(CH2)r—H, in which case R3 is W; and W is selected from sulphonic acid and sulphonate. The invention also provides a method for saturation labeling of a protein with a fluorescent dye so as to label all available target amino acid, suitably cysteine, residues in the protein, thereby giving a single population of labeled protein molecules.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.