Facile method and apparatus for the analysis of biological macromolecules in two dimensions using common and familiar electrophoresis formats
US7517442B1 · kind B1 · utility
Assignee
Inventor
Key dates
| Filing date | Aug 4, 2000 |
| Grant date | Apr 14, 2009 |
| Priority date | — |
| Expiry date | Sep 14, 2020 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44795
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An ensemble of components and methods are disclosed for utilizing two-dimensional electrophoresis in polyacrylamide or related polymer gels using common, existing and familiar electrophoresis formats and equipment. The disclosed invention makes two-dimensional electrophoresis convenient and easy to use for individuals already using vertical “mini-gel” type systems. The invention discloses the combination of pre-cast disposable gels for both first and second separation dimensions using novel support devices and cassettes that simplify the difficult multiple sample handling and processing steps inherent in ordinary two-dimensional electrophoresis methods. Devices and methods are disclosed in said invention that provide exceptional gel to gel reproducibility.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.