Reagents and methods for improving reproducibility and reducing mispriming in PCR amplification
US7517977B2 · kind B2 · utility
6Cited by
13References
19Claims
0Family size
Assignee
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Key dates
| Filing date | Oct 17, 2005 |
| Grant date | Apr 14, 2009 |
| Priority date | — |
| Expiry date | Feb 16, 2026 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/686
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An additive for preventing mispriming in polymerase chain reaction (PCR) amplifications and assays comprising a hairpin oligonucleotide having a stem duplex greater than six nucleotides in length and a stabilized stem terminus. The additive improves PCR amplifications, including LATE-PCR amplifications when added to initial amplification reaction mixtures. It can be included in oligonucleotide sets and in kits for PCR amplification and assays.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.