Cell lysis by heating-cooling process through endothermic reaction
US7521246B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 19, 2006 |
| Grant date | Apr 21, 2009 |
| Priority date | — |
| Expiry date | Jul 8, 2026 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/25
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided is a cell lysis method including: preparing a cell sample to be lysed; heating the cell sample; and cooling the cell sample by causing an endothermic reaction near the cell sample. According to the method, cell lysis can be simply and conveniently performed without regard to location and without additional devices since a separate energy source is not required and the apparatus is portable. In particular, when cell lysis is performed in a biochip using a small amount of sample, a greater cell lysis effect can be obtained. In addition, cell lysis efficiency is significantly improved, compared to when only heating is performed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.