Polyclonal-monoclonal ELISA assay for detecting N-terminus proBNP
US7527939B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 13, 2006 |
| Grant date | May 5, 2009 |
| Priority date | — |
| Expiry date | Mar 13, 2026 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/80
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A specific and sensitive in vitro ELISA assay and diagnostic test kit is disclosed for determining levels of NT-proBNP protein in a variety of bodily fluids, non-limiting examples of which are blood, serum, plasma, urine and the like. The NT-proBNP ELISA assay test employs the sandwich ELISA technique to measure circulating NT-proBNP in human plasma. In order to obtain antibodies with specific binding properties for targeted amino acid sequences within human proBNP, recombinant human proBNP (or rhproBNP) was expressed and purified for use as an immunogen. Polyclonal antibodies (PAb) to specific amino acid sequences were subsequently purified from goat serum by sequential affinity purification. Monoclonal antibodies were raised against specific polypeptides. Recombinant human NT-proBNP (or rhNT-proBNP) was expressed and purified in order to obtain material for use in calibration of a quantitative method for measurement of human NT-proBNP.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.