Nucleic acid hybridization methods
US7531306B2 · kind B2 · utility
1Cited by
4References
51Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Nov 10, 2004 |
| Grant date | May 12, 2009 |
| Priority date | — |
| Expiry date | Feb 17, 2025 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6837
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed herein are methods of destabilizing double-stranded nucleic acid hybridization using an enzyme comprising DNA N-glycosylase activity. Also disclosed herein is the detection of a double-stranded target DNA wherein the hybridization of duplex strands has been at least partially disrupted thereby permitting invasion of a probe strand. Also disclosed herein are methods of using an enzyme comprising DNA N-glycosylase activity to generate single-stranded circular nucleic acids.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.