Fluorescence polarization assay to detect protease cleavage
US7531317B2 · kind B2 · utility
3Cited by
5References
33Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Nov 24, 2004 |
| Grant date | May 12, 2009 |
| Priority date | — |
| Expiry date | Feb 20, 2025 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/37
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention discloses a method of determining protease activity, in real time, using fluorescence polarization technology. In particular, the invention provides vectors and a method for their use, which expresses uncharacterized proteins conjugated to a fluorescence tag, which binds specifically to a fluorescent ligand. Cleavage of the recombinant protein results in a fragment of the expressed peptide and results in a change in fluorescence polarization of the fluorophore. The rate of change in fluorescence polarization can be measured in real time and is equivalent to the rate of protease cleavage.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.