Methods for producing recombinant histidine-tagged inosine nonophosphate dehydrogenase polypeptides
US7531336B2 · kind B2 · utility
0Cited by
10References
24Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 20, 2007 |
| Grant date | May 12, 2009 |
| Priority date | — |
| Expiry date | Jun 20, 2027 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/81
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to a modified IMPDH polypeptide wherein the IMPDH polypeptide has a histidine tag and where the subdomain of the IMPDH polypeptide is modified so that the rate stability of the histidine-tagged, modified IMPDH polypeptide is maintained relative to the wild-type IMPDH polypeptide. The invention also relates to a method, nucleic acid molecules, vectors, and host cells for producing such a histidine-tagged, modified IMPDH polypeptide, and to kits comprising the histidine-tagged, modified IMPDH polypeptide.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.