Liquid-gas-phase exposure reactor for cell culturing

US7531351B2 · kind B2 · utility

Assignee

• ProBioGen AG · DE

Inventors

• Uwe Marx · Postbauer-Heng, DE
• Marco Riedel · Berlin, DE
• Hikmat Bushnaq-Josting · Berlin, DE

Key dates

• Filing date: Jun 14, 2005
• Grant date: May 12, 2009
• Expiry date: May 7, 2026

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12M25/12
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Initiation of growth and cultivation of cells can be performed by introducing the cells into culture compartments of a liquid-gas-phase exposure bioreactor containing a supply chamber in which there are disposed hollow-filament membranes having an inside diameter of no larger than 5 mm, wherein an inner volume of said hollow-filament membranes forms the culture compartments. Approximately one half of the supply chamber is filled with a nutrient medium and a remainder is filled with a gas mixture. Perfusion of medium and gas is turned on simultaneously or separately. The hollow-filament membranes and the cells contained therein are cyclically exposed to the gas or liquid phase.