β-D-ribosidase activity indicators with a chromogenic substrate
US7531646B2 · kind B2 · utility
1Cited by
0References
7Claims
0Family size
Inventor
Key dates
| Filing date | Oct 24, 2002 |
| Grant date | May 12, 2009 |
| Priority date | — |
| Expiry date | Sep 5, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/34
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention provides a method of detecting enzyme activity on a solid medium. The enzyme substrate has a chromogenic portion comprising a catechol residue, in which a derivitising moiety is linked to the aromatic ring of the catechol via a bond, and an enzyme cleavable group which is attached via an ester or ether linkage to the oxygen atom derived from a hydroxyl group of the catechol residue. If the enzyme substrate contacts an enzyme capable of cleaving the enzyme cleavable groups and the cleaved compound contacts a chelatable metal ion, a substantially non-diffusable coloured precipitate is formed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.