Functional assays that use the T1R2 taste receptor to identify potential taste modulators
US7534577B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 2, 2003 |
| Grant date | May 19, 2009 |
| Priority date | — |
| Expiry date | Aug 5, 2025 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/726
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Functional assays that use hT1R2 receptor polypeptides to screen for taste modulatory compounds are provided. In preferred embodiments these assays will use cells that stably or transiently express an hT1R2 polypeptide and which further express a suitable protein, e.g. Gα15, Gα16 or gustducin. Such functional assays will screen for the effect of compound on T1R2 activity by detecting changes in cell voltage, intracellular ions, phosphorylation, second messenger levels and the like. Compounds identified in such assays are potentially useful as taste modulators. In preferred embodiments, the effect of selected compounds is evaluated further in human taste tests.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.