Methods of measuring luminogenic activity with a protected coelenterazine
US7537912B2 · kind B2 · utility
26Cited by
40References
25Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 1, 2007 |
| Grant date | May 26, 2009 |
| Priority date | — |
| Expiry date | Aug 1, 2027 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/66
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of measuring the enzymatic activity of a luciferase includes contacting a luminogenic protein, such as a luciferase, with a protected luminophore to form a composition; and detecting light produced from the composition. The protected luminophore provides increased stability and improved signal-to-background ratios relative to the corresponding unmodified coelenterazine.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.