Methods for protein refolding
US7538198B2 · kind B2 · utility
9Cited by
23References
42Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Apr 29, 2003 |
| Grant date | May 26, 2009 |
| Priority date | — |
| Expiry date | Jan 5, 2025 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/2462
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention discloses improved methods of disaggregating protein aggregates, and refolding denatured proteins, using high pressure. In particular, the present invention provides for the use of agitation, high temperature, “stepped” depressurization, dialysis and dilution under pressure to increase the speed and extent of aggregate dissolution and protein refolding.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.