Method of providing a substrate with a ready-to-use, uniformly distributed extracellular matrix
US7541187B2 · kind B2 · utility
8Cited by
16References
9Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 6, 2002 |
| Grant date | Jun 2, 2009 |
| Priority date | — |
| Expiry date | Dec 22, 2022 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/90
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Method of providing a substrate with a ready-to-use uniformly distributed extracellular matrix is disclosed. This method includes applying extracellular matrix components to a substrate area; incubating the extracellular matrix components to allow polymerization thereof; freezing the polymerized extracellular matrix on the substrate area; lyophilizing the frozen extracellular matrix on the substrate area; and allowing the lyophilized extracellular matrix to warm to room temperature. Also contemplated is a cell culture apparatus having a dried uniformly distributed extracellular matrix formed by the above-mentioned method.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.