Vacuum assisted affinity chromatography device and method
US7546779B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 26, 2007 |
| Grant date | Jun 16, 2009 |
| Priority date | — |
| Expiry date | Feb 20, 2028 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2030/381
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Sample preparation device and method particularly useful for large volume capture and small volume elution. The device comprising a manifold, a sample holder or reservoir, and a filter unit containing chromatography media such that a filtration path is established between the sample holder, the filter unit and the manifold. Upon subjecting the sample in the sample holder to a driving force such as vacuum, the sample flows through the chromatography media in the filter unit. Molecules of interest bind to the media and any unwanted molecules can be washed under vacuum mode. Elution can then be carried out by removing the filter unit and subjecting the media to a driving force such as vacuum or centrifugation. In another embodiment, molecules of interest can be eluted into a low volume centrifugal spinner directly in the manifold.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.