Peptide-based fluorescence polarization assay for detection of antibodies to Mycobacterium bovis
US7547506B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Oct 31, 2002 |
| Grant date | Jun 16, 2009 |
| Priority date | — |
| Expiry date | Aug 10, 2024 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/542
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides an assay for detection of M. bovis-infected animals. A tracer, comprising a peptide of M. bovis protein MPB70 conjugated to a fluorophore, is added to a serum sample from an animal to form a mixture. The fluorescence polarization of the mixture in then measured and compared to the fluorescence polarization of a control. The present invention further provides a tracer for use in fluorescence polarization assay to detect antibodies specific for M. bovis. The tracer comprises a peptide of M. bovis protein MPB70 conjugated to a fluorophore, such that the tracer is able to bind to antibodies specific for M. bovis to produce a detectable change in fluorescence polarization.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.