Genetically engineered pyrroloquinoline quinone dependent glucose dehydrogenase comprising an amino acid insertion

US7547524B2 · kind B2 · utility

Assignee

• Roche Diagnostics Operations, Inc. · US

Inventors

• Mara Boenitz-Dulat · Tutzing, DE
• Jessica Laggerbauer · Birkenfeld, DE
• Rainer Schmuck · Benediktbeuern, DE
• Peter Kratzsch · Penzberg, DE
• Wolfgang-Reinhold Knappe · Ludwigshafen, DE

Key dates

• Filing date: Jul 19, 2005
• Grant date: Jun 16, 2009
• Expiry date: Jul 19, 2025

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/0006
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH) comprising an amino acid insertion between positions 428 and 429 as corresponding to the amino acid sequence known from Acinetobacter calcoaceticus, to genes encoding such variant s-GDH, to proteins of such s-GDH variants with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugars, especially of glucose in a sample.