Metaphase donor cells for effective nuclear reprogramming in mammals
US7547818B2 · kind B2 · utility
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4Claims
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Key dates
| Filing date | Mar 25, 2004 |
| Grant date | Jun 16, 2009 |
| Priority date | — |
| Expiry date | Sep 23, 2025 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/873
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides methods of producing a clone non-human mammalian nuclear transfer (NT) embryo and methods for producing a cloned non-human mammal. Embodiments of the methods include introducing doner genetic material into a metaphase I oocyte; introducing donor genetic material into a non-enucleated oocyte; introducing donor genetic material obtained from a donor cell that is a metaphase into an oocyte; introducing donor genetic material into an oocyte, and naturally activating the oocyte or the NT embryo; and introducing donor genetic material obtained from a donor cell that is at late G1 phase into anoocyte.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.