Crystal structure of SNAP-25 in complex with botulinum neurotoxin and uses thereof
US7553651B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 29, 2006 |
| Grant date | Jun 30, 2009 |
| Priority date | — |
| Expiry date | Aug 1, 2026 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2299/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides a crystallized form of the E223Q Y365F mutant of the light chain of botulinum neurotoxin serotype A (BoNT/A) protein as well as the atomic structure of this mutant protein. The present invention also provides crystals of the E223Q Y365F mutant of the BoNT/A light chain in complex with the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) domain of the SNAP-25 (synaptosome-associated protein-25 kDa) protein as well as the atomic structure of this complex. The present invention further provides a method of determining whether an agent mimics a region of the SNAP-25 protein that interacts with BoNT/A. With this method, a three-dimensional representation of the SNAP-25 region is used to computationally compare the agent with the three-dimensional representation of the SNAP-25 region.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.