Mutant strains of Pseudomonas fluorescens and variants thereof, methods for their production, and uses thereof in alginate production

US7553656B2 · kind B2 · utility

Assignee

• FMC Biopolymer AS · NO

Inventors

• Martin Gimmestad · Trondheim, NO
• Havard Sletta · Trondheim, NO
• Karuna Ponniah Karunakaran · Vancouver, CA
• Karianne Bakkevig · Trondheim, NO
• Helga Ertesvag · Trondheim, NO
• Trond Erling Ellingsen · Trondheim, NO
• Gudmund Skjak-Braek · Trondheim, NO
• Svein Valla · Vikhammer, NO

Key dates

• Filing date: Jul 24, 2003
• Grant date: Jun 30, 2009
• Expiry date: Jul 24, 2023

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12R2001/39
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

It is described biologically pure bacterial cultures of mutant strains of Pseudomonas fluorescens, which produces large amounts of alginate. The alginate may contain a certain determined content of mannuronate and guluronate residues, possible presence and determined level of acetyl groups in the alginate, and a desired molecular weight of the alginate. Also high yielding mutants with regulation of alginate production, is described. The invention further provides methods for producing new mutant strains of Pseudomonas fluorescens and variants thereof, and use the resulting strains in alginate production.