Motion resolved molecular sequencing

US7556922B2 · kind B2 · utility

Assignee

• The Board of Trustees of the Leland Stanford Junior University · US

Inventors

• Steven M. Block · Redwood City, US
• William J. Greenleaf · Menlo Park, US

Key dates

• Filing date: Mar 21, 2007
• Grant date: Jul 7, 2009
• Expiry date: May 11, 2027

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6874
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods are provided for determining the sequence of a polynucleotide by tracking the motion of a nucleotide-selective, processive enzyme along a template, in a reaction mixture that alters the enzyme movement in a base specific manner. The movement trace of the enzyme is collected, and arranged in temporal order. The enzyme movement traces are correlated with the reaction conditions, and from these characteristic signals, the nucleotide sequence is derived.