Methods and compositions for use in preparing siRNAs
US7556944B2 · kind B2 · utility
11Cited by
0References
14Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Apr 30, 2003 |
| Grant date | Jul 7, 2009 |
| Priority date | — |
| Expiry date | Nov 28, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2330/30
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods and compositions for producing siRNAs, e.g., in the form of a d-siRNA composition, from dsRNAs are provided. In the subject methods, a dsRNA is contacted with a composition that includes an activity that cleaves dsRNA into siRNAs, where the composition efficiently cleaves dsRNA into siRNAs. siRNAs produced by the subject methods find use in a variety of applications, particularly in applications where the specific reduction or silencing of a gene is desired. Also provided are kits for use in practicing the subject invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.