Foamy virus mutant reverse transcriptase
US7560117B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 22, 2002 |
| Grant date | Jul 14, 2009 |
| Priority date | — |
| Expiry date | Aug 8, 2022 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2740/17022
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a highly processive reverse transcriptase having DNA polymerase activity and substantially reduced protease activity. More specifically, the invention relates to an isolated reverse transcriptase from foamy virus comprising a substantially inactivated protease. The invention also relates to vectors containing the gene and hosts transformed with the vector of the invention. Further, the invention relates to a method for producing reverse transcriptase having DNA polymerase activity and substantially reduced protease activity by expressing the reverse transcriptase genes of the present invention in a recombinant host. Methods are also provided for producing cDNA from polynucleotides using the highly processive reverse transcriptase of the invention. Kits for the preparation of cDNA from RNA comprising the highly processive reverse transcriptase of the invention are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.