Methods of capturing, detecting and quantifying RNA:DNA hybrids and a modified RNase H useful therein
US7560232B2 · kind B2 · utility
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24Claims
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Key dates
| Filing date | Dec 18, 2003 |
| Grant date | Jul 14, 2009 |
| Priority date | — |
| Expiry date | Sep 18, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6827
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for specific RNA capture, detection and quantification are presented utilizing a protein that selectively binds RNA:DNA hybrids, preferably an RNase H that is modified to reduce degradation of the nucleic acid molecules and enhance specific detection of mixed RNA:DNA nucleic acid hybrids. Labeling of the RNA and/or amplification is not required to perform these methods. Modified RNase H enzymes useful in such methods are disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.