Allele specific primer extension

US7560254B2 · kind B2 · utility

Assignee

• GE Healthcare Bio-Science AB · US

Inventors

• Anup Sood · Flemington, US
• Shiv Kumar · Montgomery, US
• Carl W. Fuller · Cleveland Heights, US
• John Richard Nelson · Fryeburg, US
• John Macklin · Wenonah, US

Key dates

• Filing date: Aug 29, 2003
• Grant date: Jul 14, 2009
• Expiry date: Nov 9, 2024

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6858
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method of characterizing a nucleic acid sample is provided that includes the steps of: (a) conducting a DNA polymerase reaction that includes the reaction of a template, an allele specific primer, at least one terminal phosphate-labeled nucleotide, DNA polymerase, and optionally an enzyme having 3′→5′ exonuclease activity when the primer is non-hydrolyzable, which reaction results in the production of labeled polyphosphate; (b) permitting the labeled polyphosphate to react with a phosphatase to produce a detectable species; (c) detecting the detectable species; and (d) characterizing the nucleic acid sample based on such detection.