Automated process for detecting the presence of a target nucleic acid in a sample

US7560256B2 · kind B2 · utility

Assignee

• GEN-PROBE INCORPORATED · US

Inventors

• Kelly G. Ammann · Longmont, US
• Ralph E. Burns · Boulder, US
• Ernest V. Hansberry · Evergreen, US
• Glenn A. Horner · Boulder, US
• Cheryl A. Jakub · Golden, US
• John E. Kling · San Diego, US
• Donald J. Nieglos · Superior, US
• Robert E. Schneider · Erie, US
• Robert J. Smith · Brookline, US

Key dates

• Filing date: Jun 29, 2005
• Grant date: Jul 14, 2009
• Expiry date: Jun 3, 2026

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/2575
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

An automated process for detecting the presence of a target nucleic acid in a sample. The process includes separating the target nucleic acid from other material present in the sample at a first station, transferring the separated target nucleic acid from the first station to a second station using a rotatable transport mechanism, combining the transferred target nucleic acid and reagents for performing an amplification reaction at the second station, and performing an amplification reaction. A product of the amplification reaction is detected as an indication of the presence of the target nucleic acid in the sample.