Assay methods using chemiluminescent detection of peroxidase
US7560556B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 4, 2005 |
| Grant date | Jul 14, 2009 |
| Priority date | — |
| Expiry date | Apr 26, 2026 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/908
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods for generating chemiluminescence rapidly by reaction of at least one compound comprising a C—C double bond substituted at one carbon with two sulfur atom-containing groups with a peroxidase enzyme and a peroxide are disclosed. The chemiluminescence thus produced can be used as a detectable signal in assays for peroxidase enzymes or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs. Use of the methods provides rapid signal generation, achieving a plateau intensity in under one minute. The substrate can be provided in a composition which demonstrates unexpectedly long storage stability.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.