Synthetic nucleic acids for expression of encoded proteins
US7561972B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 31, 2008 |
| Grant date | Jul 14, 2009 |
| Priority date | — |
| Expiry date | Jul 31, 2028 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16B30/00
- WIPO fieldComputer technology
- WIPO sectorElectrical engineering
Abstract
A method of designing a polynucleotide sequence encoding a polypeptide sequence of a predetermined polypeptide is provided. A frequency lookup table corresponding to an expression system is obtained. The table comprises a plurality of sequence elements and a plurality of frequency ranges, each frequency range for a corresponding sequence element. Each frequency range is a range of frequencies with which a corresponding sequence element can occur in a polynucleotide. The polynucleotide sequence is defined using the frequency lookup table by determining, for each respective sequence element in the frequency lookup table, whether the respective sequence element encodes a portion of the polypeptide sequence. When the respective sequence element encodes a portion of the polypeptide sequence, the sequence element is incorporated into the polynucleotide at a frequency of occurrence that is within the frequency range specified for the respective sequence element in the lookup table. The polynucleotide sequence is then outputted.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.