Reconstituted histone methyltransferase complex and methods of identifying modulators thereof
US7563589B2 · kind B2 · utility
43Cited by
1References
15Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 27, 2005 |
| Grant date | Jul 21, 2009 |
| Priority date | — |
| Expiry date | May 27, 2025 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/91011
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a reconstituted complex including EED, EZH2 and SUZ12 wherein the reconstituted complex has histone methyltransferase (HMTase) activity for lysine 27 of histone H3 (H3-K27). The reconstituted complex may further include RbAp48, AEBP2 or both. Also disclosed are methods of producing the reconstituted complex, methods of identifying compounds that inhibit the HTMase activity of the reconstituted complex and methods of identifying candidate compounds for treating cancer. Reagents and kits including the reconstituted complex are further provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.