Process for separating and isolating xanthines, individual polar protic monomers, and polar protic oligomers

US7566401B2 · kind B2 · utility

Inventors

• Mark A. Kelm · East Stroudsburg, US
• Christopher J. Johnson · London, GB
• Rebecca J. Robbins · Budd Lake, US

Key dates

• Filing date: Oct 3, 2005
• Grant date: Jul 28, 2009
• Expiry date: Nov 13, 2027

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B01J2220/54
• WIPO field: Chemical engineering
• WIPO sector: Chemistry

Abstract

An improved process for separating and isolating individual polar protic monomer(s) and/or oligomer(s) on the basis of degree of polymerization. A liquid sample containing polar protic monomer(s) and/or oligomer(s) is introduced into a liquid chromatography (LC) column packed with a polar bonded stationary chromatographic phase. The individual polar protic monomer(s) and/or oligomer(s) are separated via a binary mobile phase elution. One or more individual fractions containing the monomer(s) and/or oligomer(s) are eluted. The polar protic monomer(s) and/or oligomer(s) may be proanthocyanidins, hydrolyzable tannins, oligosaccharides, oligonucleotides, peptides, acrylamides, polysorbates, polyketides, poloxamers, polyethylene glycols, polyoxyethylene alcohols or polyvinyl alcohols. The binary mobile phase comprises an A phase consisting essentially of a polar aprotic solvent and a B phase consisting essentially of a polar protic solvent.A process for separating and isolating xanthine(s) (e.g., caffeine and theobromine) from polar protic monomer(s) and/or oligomer(s). A liquid sample containing xanthine(s) and polar protic monomer(s) and/or oligomer(s) is introduced into an LC column …