Compositions and methods for identifying agents that alter expression of survivin
US7569221B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Nov 12, 2007 |
| Grant date | Aug 4, 2009 |
| Priority date | — |
| Expiry date | Nov 12, 2027 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/142
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided are compositions and methods for identifying agents that can modulate expression of the human survivin gene. The compositions include eukaryotic cells that contain a human survivin promoter sequence operably linked to a reporter gene. The method comprises determining whether a test agent can modulate transcription from a human survivin promoter sequence by adding a test agent to eukaryotic containing a human survivin promoter sequence operably linked to a reporter gene, measuring expression of the reporter gene and comparing expression of the reporter gene from to a control, wherein an increase or decrease of expression of the reporter gene relative to the control is indicative that the test agent can modulate transcription from a human survivin promoter. The method also comprises the use of SPlucTg mice for preclinical drug identification, including testing of candidate drug toxicity and efficacy.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.